Regulatory

Part:BBa_J72005:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)


{Ptet} promoter in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1091F/R on pSB1A2-r0040    (~100 bp, EcoRI/BamHI)
Sub into pBca9145-Bca1089        (EcoRI/BamHI)
Product is pBca9145-Bca1091
----
ca1091F	Forward EcoRI/BglII for Ptet part
ttctggaattcatgAGATCTtccctatcagtgatagag
ca1091R	Reverse BamHI for Ptet part
GtttGGATCCgtgctcagtatctctatc

References